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KMID : 1059519860300020216
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Journal of the Korean Chemical Society
1986 Volume.30 No. 2 p.216 ~ p.223
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Separation of Optical Isomers of DNS-Amino Acids in High-Performance Liquid Chromatography
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Lee Sun-Haing
Oh Tae-Sub
Park Kyung-Sug
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Abstract
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Separation of optical isomers of DNS derivatized amino acids by a reversed-phase high-performance liquid chromatography has been studied by adding a complex of an optically active amino acid (L-arginine) with the metal ion (Cu(II), Zn(II), Cd(II), Ni(II)) to the mobile phase. The separations are affected by the concentrations of acetonitrile, chelate and buffer. They are also affected by the pH and the kinds of metal and buffer. A separation mechanism, which is based on steric effect of the ligand exchange reaction for the formation of ternary complexes by the D,L-DNS-amino acids and the chiral additive associated with the stationary phase, is proposed to interpret the elution behaviors of D, L-dansyl-amino acids.
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